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技術(shù)文章ARTICLE

恒遠(yuǎn)生物魚蛋白酶（Protease） ELISA試劑盒引用文獻(xiàn)

點(diǎn)擊次數(shù)：12664 發(fā)布時間：2020/12/21 16:34:59

【文獻(xiàn)標(biāo)題】Improved utilization of soybean meal through fermentation with commensal Shewanella sp. MR-7 in turbot (Scophthalmus maximus L.)

【作者】Chaoqun Li，Beili Zhang，Xin Wang，et.al

【作者單位】中海洋大學(xué)（Ocean University of China）

【文獻(xiàn)中引用產(chǎn)品】

魚蛋白酶（Protease） elisa試劑盒

【關(guān)鍵詞】Turbot, Shewanella sp. MR-7, Soybean meal, Fermentation, Intestinal health, Intestinal microbiota

【DOI】https://doi.org/10.1186/s12934-019-1265-z

【影響因子(IF)】7.40

【出版期刊】《Microbial Cell Factories》

【產(chǎn)品原文引用】

Parameter optimization of Shewanella sp. MR‑7

Te isolated strain of Shewanella sp. MR-7 was cultured in 2216E medium (Qingdao Haibo) ａnd shaken at 220 rpm at 25, 30, 35, 37 ａnd 40 °C to determine the efects of temperature on the growth of Shewanella sp. MR-7. Te Shewanella sp. MR-7 was also cultured in 2216E medium at pH 5.0, 6.0, 7.0, 8.0, 9.0 to examine the efect of pH on its growth. Te OD600 of the culture was taken periodically. Te infuences of temperature ａnd pH on the production of protease by Shewanella sp. MR-7 were determined by enzyme-linked immunosorbent assay (ELISA) using protease ELISA kits (HengYuan Biological Technology Co., Ltd, Shanghai, China).